Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Saccharomyces cerevisiae Transcript Isoform mapping

ABSTRACT: Extensive transcriptional heterogeneity revealed by isoform profiling Application of TIF-Seq (Transcript IsoForm Sequencing) to S.cerevisiae. The method was applied to simultaneously identify the 5' capped mRNA transcription start site and the 3' polyadenylation site in different conditions: WT cells grown in glucose media [ypd, 2 biological replicates (bio) and 3 independent library preparations, technical replicates(lib)], WT cells grown in galactose media [ypgal, 4 biological replicates (bio) and 3 independent library preparations, technical replicates(lib)]. A modified protocol designed to enrich in long mRNA molecules was performed for WT cells grown in glucose media [ypd, 2 biological replicates (bio)] and in galactose media [ypgal, 2 biological replicates (bio)] conditions. Finally, control samples performed with a modified protocol designed to identify non-capped but polyadenylated molecules was performed in WT cells grown both in glucose (nypd) and galactose (nypgal) media.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Wu Wei 

PROVIDER: E-GEOD-39128 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Extensive transcriptional heterogeneity revealed by isoform profiling.

Pelechano Vicent V   Wei Wu W   Steinmetz Lars M LM  

Nature 20130424 7447

Transcript function is determined by sequence elements arranged on an individual RNA molecule. Variation in transcripts can affect messenger RNA stability, localization and translation, or produce truncated proteins that differ in localization or function. Given the existence of overlapping, variable transcript isoforms, determining the functional impact of the transcriptome requires identification of full-length transcripts, rather than just the genomic regions that are transcribed. Here, by jo  ...[more]

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