Saccharomyces cerevisiae 3' poly(A) site mapping

ABSTRACT: The use of alternative polyadenylation sites is common and affects the post-transcriptional fate of mRNA, including its stability, localization, and translation. Here we present a method for genome-wide and strand-specific mapping of poly(A) sites and quantification of RNA levels at unprecedented efficiency by using an on-cluster dark T-fill procedure on the Illumina sequencing platform. Our method outperforms former protocols in quality and throughput, and reveals new insights into polyadenylation in Saccharomyces cerevisiae. Experimental benchmark of five different protocols (3tfill, bpmI, internal, rnaseq and yoon) for genome-wide identification of polyadenylation sites in Saccharomyces cerevisiae and transcript quantification. RNA was extracted from WT cells grown in glucose (ypd) or g

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Aino Järvelin 

PROVIDER: E-GEOD-40110 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress