Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation

ABSTRACT: We used genome-scale modeling and multi-omics (transcriptomics, proteomics, and metabolomics) analysis to assess metabolic features that are critical for macrophage activation. We constructed a genome-scale metabolic network for the RAW 264.7 cell line to determine metabolic modulators of activation. Metabolites well-known to be associated with immunoactivation (glucose and arginine) and immunosuppression (tryptophan and vitamin D3) were among the most critical effectors. Intracellular metabolic mechanisms were assessed, identifying a suppressive role for de-novo nucleotide synthesis. Finally, underlying metabolic mechanisms of macrophage activation are identified by analyzing multi-omic data obtained from LPS-stimulated RAW cells in the context of our flux-based predictions. Two condition (flagellin and LPS) time course exposure of RAW 264.7 cell line at 1, 2, 4, and 24 hours. Two replicates for each condition and time point. All conditions compared to a pool of untreated cells at a 0 hour time point.

ORGANISM(S): Mus musculus

SUBMITTER: Bryan Frank 

PROVIDER: E-GEOD-39785 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Json Xml
altmetric image

Publications

Macrophages are central players in immune response, manifesting divergent phenotypes to control inflammation and innate immunity through release of cytokines and other signaling factors. Recently, the focus on metabolism has been reemphasized as critical signaling and regulatory pathways of human pathophysiology, ranging from cancer to aging, often converge on metabolic responses. Here, we used genome-scale modeling and multi-omics (transcriptomics, proteomics, and metabolomics) analysis to asse  ...[more]

Similar Datasets

Transcriptomics LPS treatment of RAW2647 macrophages
2012-01-24 | E-GEOD-35237 | biostudies-arrayexpress
Unknown Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation
2012-08-03 | GSE39785 | GEO
Proteomics,Multiomics Model-driven analysis of metabolic immunomodulators of macrophage activation
2015-11-11 | PXD003055 | Pride
Unknown LPS treatment of RAW2647 macrophages
2012-01-24 | GSE35237 | GEO
Unknown Pooled CRISPR Screening Identifies m6A as a Positive Regulator of Macrophage Activation [RNA-seq]
2021-04-30 | GSE162248 | GEO
Metabolomics,Multiomics Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation
2012-09-05 | MTBLS23 | MetaboLights
Transcriptomics A new Rrf2 repressor family member, SaiR, controls transcription of spxA2 encoding a regulator of the oxidative stress response in Bacillus anthracis.
2014-05-21 | E-GEOD-57851 | biostudies-arrayexpress
Metabolomics Model-driven multi-omic data analysis elucidates metabolic immunomodulators of macrophage activation
2014-06-25 | ST000084 | MetabolomicsWorkbench
Unknown Sequencing of primary mouse machrophage and Raw 264.7 macrophage cell skewed in M1 or M2 conditions
2019-04-30 | GSE113836 | GEO
Unknown Exosomal miRNA signatures of RAW 264.7 cells
2023-04-30 | GSE149630 | GEO