A new Rrf2 repressor family member, SaiR, controls transcription of spxA2 encoding a regulator of the oxidative stress response in Bacillus anthracis.

ABSTRACT: Spx, a member of the ArsC (arsenate reductase) protein family, is highly conserved in Gram-positive bacteria, where it interacts with RNA polymerase to activate transcription in response to toxic oxidants.  In Bacillus anthracis, resistance to oxidative stress requires the activity of two paralogous forms of Spx, SpxA1 and SpxA2.   Suppressor mutations were identified in spxA1 mutant cells that conferred resistance to hydrogen peroxide.  The mutations, which reside in saiR within the operon that also contains the spxA2 gene, caused derepression of spxA2 transcription. The product of saiR is a member of the Rrf2 family of transcriptional repressors.  Derepression of spxA2 in a saiR mutant required SpxA2, indicating an autoregulatory mechanism of spxA2 transcriptional activation.  Reco

ORGANISM(S): Bacillus anthracis

SUBMITTER: Bryan Frank 

PROVIDER: E-GEOD-57851 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress