Mouse bone marrow cells transfected with mmu-miR-142-3p mimic oligo.

ABSTRACT: Tumor progression is accompanied by an altered myelopoiesis that causes the accumulation of cells inhibiting anti-tumor T lymphocytes. We previously reported that immunosuppressive cells can be generated in vitro from bone marrow cells (BM) after four days GM-CSF and IL-6 treatment. Here, we describe that miR-142-3p down-regulation directs macrophage differentiation and determines the acquisition of their immunosuppressive function in cancer. Enforced miR over-expression impaired monocyte to macrophage transition both in vitro and in vivo. Conversely, forced miR down-regulation promoted the generation of immunosuppressive macrophages even during G-CSF-induced granulocytic differentiation. To identify how miR-142-3p regulates MDSC generation and activity, we analyze the gene expression of B

ORGANISM(S): Mus musculus

SUBMITTER: Stefania Bortoluzzi 

PROVIDER: E-GEOD-39803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress