Effects of manR disruption and overexpression on Aspergillus oryzae gene expression

ABSTRACT: Fungal endo-M-NM-2-mannanases (M-NM-2-mannanases) are widely used as industrial enzymes; however, no transcriptional regulator of M-NM-2-mannanases has been identified in fungi or other eukaryotic cells to date. To identify a transcriptional regulator of M-NM-2-mannanases in Aspergillus oryzae, a gene-disruptant library of transcriptional regulators was screened for mutants exhibiting reduced M-NM-2-mannanase activity by using konjac glucomannan as the substrate, and ManR, a Zn(II)2Cys6 type DNA binding protein was identified. Moreover, a manR-overexpressing strain showed significantly increased M-NM-2-mannanase activity. DNA microarray analysis of the manR-disruptant strain and the manR-overexpressing strain further indicated that when konjac glucomannan is used as the carbon source, ManR

ORGANISM(S): Aspergillus oryzae RIB40

SUBMITTER: Masahiro Ogawa 

PROVIDER: E-GEOD-40590 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress