DDM1 and RdDM are the major regulators of transposon DNA methylation in Arabidopsis

ABSTRACT: Eukaryotic DNA methylation is found in silent transposable elements and active genes. Nucleosome remodelers of the DDM1/Lsh family are thought to be specifically required to maintain transposon methylation, but the reason for this is unknown. Here, we find that a chromatin gradient that extends from the most heterochromatic transposons to euchromatic genes determines the requirement of DDM1 for methylation maintenance in all sequence contexts. We also show that small RNA-directed DNA methylation (RdDM) is inhibited by heterochromatin and absolutely requires the nucleosome remodeler DRD1. DDM1 and RdDM independently mediate nearly all transposon methylation, which is catalyzed by the methyltransferases MET1 (CG), CMT3 (CHG), DRM2 (CHH) and CMT2 (CHH), and collaborate to repress transpositio

ORGANISM(S): Arabidopsis thaliana

SUBMITTER: Toshiro Nishimura 

PROVIDER: E-GEOD-41302 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress