Analysis of Unique and Overlapping Patterns of Gene Expression After Treatment of Zebrafish Embryos with Estradiol and/or Dioxin

ABSTRACT: To increase the utility of the zebrafish gene expression bioassay for assessing EDC effects on multiple gene targets and tissue types, and to expand our understanding of genetic overlap between estrogen receptor (ER) and arylhydrocarbon receptor (AhR) mediated signaling pathways, we conducted microarray analysis of zebrafish embryos exposed to estradiol and dioxin alone or in combination. Of >16,000 probe sets on the array, 34 were regulated by estradiol (E2), 86 by dioxin (TCDD) and 109 by E2+TCDD (as chosen by >2-fold change and p<0.1). Of 62 genes selected for verification by QPCR, 14 genes, or 22% were reproducibly up- or down-regulated, offering potential additional target genes for screening of estrogen- and dioxin-like EDC. The majority of these successful hits, 11, were TCDD-respon

ORGANISM(S): Danio rerio

SUBMITTER: Adam Gower 

PROVIDER: E-GEOD-42084 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress