Genome wide DNA methylation sequencing of ischemic skeletal muscle from normal, hyperlipidemic and type 2 diabetic mice

ABSTRACT: Objective: To define the role of epigenetics, particularly DNA methylation in adaptive vascular growth in hyperlipidemic and type 2 diabetic mouse models of hind limb ischemia Methods: Unilateral hindlimb ischemia was induced by ligating femoral artery proximal to the bifurcation of superficial and deep femoral artery. DNA was isolated from ischemic muscles collected at day 7 after ischemia induction using DNeasy Tissue kit (Qiagen). 5 µg of genomic DNA was sheared into small fragments with a mean size of 150 bp by using a Covaris™ S2 sonicator System. Quality of the fragmentation was analyzed with Bioanalyzer. Fragmented DNA samples were used for preparation of DNA fragment libraries and sequenced on the SOLiD4 sequencing instrument on one flow cell for 50 bp reads. The sequencing read

ORGANISM(S): Mus musculus

SUBMITTER: Mohan Babu 

PROVIDER: E-GEOD-42092 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress