Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from 0.15M and 0.7M-fed wild-type and ChREBP mutant, third instar Drosophila larval fat bodies (FBs)

ABSTRACT: Chronic high sugar feeding induces obesity, hyperglycemia, and insulin resistance in flies and mammals. These phenotypes are controlled by the fat body, a liver- and adipose- like tissue in Drosophila flies. To gain insight into the mechanisms underlying the connection between diet and insulin sensitivity, we used Illumina RNA-seq to profile gene expression in fat bodies isolated from chronically high sugar fed, wandering (post-prandial) third instar wild type larvae w(L3). These data were compared to control-fed wild-type wL3 fat bodies as well as those expressing transgenic interfering RNA (i) targeting CG18362 (Mio/dChREBP) in the fat body on both diets. Female VDRC w1118, cgGAL4, UAS-Dcr2 or UAS-ChREBPi(52606), cgGAL4, UAS-Dcr2 wandering third instar larvae were fed control (0.15M) or high (0.7M) sucrose and fat bodies isolated for RNA extraction.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Thomas Baranski 

PROVIDER: E-GEOD-43734 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Role of fat body lipogenesis in protection against the effects of caloric overload in Drosophila.

Musselman Laura Palanker LP   Fink Jill L JL   Ramachandran Prasanna Venkatesh PV   Patterson Bruce W BW   Okunade Adewole L AL   Maier Ezekiel E   Brent Michael R MR   Turk John J   Baranski Thomas J TJ  

The Journal of biological chemistry 20130125 12

The Drosophila fat body is a liver- and adipose-like tissue that stores fat and serves as a detoxifying and immune responsive organ. We have previously shown that a high sugar diet leads to elevated hemolymph glucose and systemic insulin resistance in developing larvae and adults. Here, we used stable isotope tracer feeding to demonstrate that rearing larvae on high sugar diets impaired the synthesis of esterified fatty acids from dietary glucose. Fat body lipid profiling revealed changes in bot  ...[more]

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