Expression profile of the fat body from bigmax mutant and wildtype third instar Drosophila melanogaster larvae

ABSTRACT: To identify genes differentially expressed in the fatbody of Drosphila melanogaster bigmax mutants, a loss-of-function allele was generated by P-element mobilization. Mutant and wildtype first instar larvae were raised on two different sources of food, control and high-sugar media. When the animals reached the wandering third instar stage, animals were sacrificed and their fat bodies dissected. Total RNA was extracted, labeled fluorescently and hybridized competitively to Agilent's 4x44K Drosophila Gene Expression Microarrays. On each array, three different samples were analyzed: 1. wildtype animals raised on control food, 2. wildtype animals raised on high-sugar food and 3. bigmax mutant animals raised on high-sugar food.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Thomas Sandmann 

PROVIDER: E-MTAB-699 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress