Transcription profiling of human actute monocytic leukemia cell line THP-1 cells incubated at 37 or 32 degrees C for 24h reveals moderate hypothermia produces substantial changes in cellular gene expression, in categories potentially of importance to systemic function.

ABSTRACT: Background: Moderate hypothermia (32oC for 12 – 72 hours) has therapeutic applications, but the mechanisms by which it affects cellular function are unclear. We tested the hypothesis that moderate hypothermia produces broad changes in gene expression by human cells at the level of mRNA. Methods: Acute monocytic leukemia THP-1 cells were incubated under control conditions (37oC) or moderate hypothermia (32oC) for 24 hours. Cellular mRNA was extracted and a cold stress response was confirmed by examining the expression of the cold-inducible gene CIRBP by reverse transcription PCR (RT-PCR). Gene expression analysis was performed on seven sets of samples with Affymetrix U133A chips, using established statistical methods. Sequences were considered to be affected by cold if they showed s

ORGANISM(S): Homo sapiens

SUBMITTER: Larry Sonna 

PROVIDER: E-GEOD-4425 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress