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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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RNA-Seq and RNA Polymerase II ChIP-Seq of mouse spermatogenesis

ABSTRACT: To characterize gene expression in spermatogenesis we sequenced eight mRNA samples from testes of juvenile mice aged between 6 and 38 days post partum. We then classified gene expression profiles and found over 1,000 meiotically-expressed protein-coding genes that have not been previously reported in relation to spermatogenesis. Furthermore, we developed an in silico de-convolution approach to estimate cell type-specific gene expression from temporal gene expression profiles and applied it to our dataset. To further characterize transcription during spermatogenesis, we determined RNA polymerase II (RNAPII) distribution along the genome using ChIP-Seq. mRNA sequencing at 8 time points during spermatogenesis was used to characterize differential gene expression; RNA Pol II ChIP-Seq at two time points was used to estimate RNA Pol II accumulation at promoters.

ORGANISM(S): Mus musculus

SUBMITTER: Gennady Margolin

PROVIDER: E-GEOD-44346 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Integrated transcriptome analysis of mouse spermatogenesis.

Margolin Gennady G Khil Pavel P PP Kim Joongbaek J Bellani Marina A MA Camerini-Otero R Daniel RD

BMC genomics 20140118

<h4>Background</h4>Differentiation of primordial germ cells into mature spermatozoa proceeds through multiple stages, one of the most important of which is meiosis. Meiotic recombination is in turn a key part of meiosis. To achieve the highly specialized and diverse functions necessary for the successful completion of meiosis and the generation of spermatozoa thousands of genes are coordinately regulated through spermatogenesis. A complete and unbiased characterization of the transcriptome dynam ...[more]

PMID: 24438502

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