Analysis of Drosophila salivary glands and Kc cells with depleted levels of linker histone H1 (Illumina smRNA-Seq]

ABSTRACT: Salivary glands or larval ovaries were isolated from transgenic flies expressing RNAi targeting Nautilus (control) or linker histone H1 using a Tub-Gal4 driver. ~200 larvae were used to isolate salivary glands or ovaries, independently. Total RNA was isolated using Trizol reagent following manufacturer's guidelines. Then 5 M-BM-5g of total RNA was separated on a polyacrylamide gel, and 18-29 nt small RNAs were isolated for cloning.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Sandeep Wontakal 

PROVIDER: E-GEOD-44399 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress