Laser Capture Microdissection isolation of preovulatory granulosa cells from WT and bERKO ovaries

ABSTRACT: Determining the spatial and temporal expression of genes involved in the ovulatory pathway is critical for the understanding of the role of each estrogen receptor in the modulation of folliculogenesis and ovulation. Estrogen receptor (ER) b is highly expressed in ovarian granulosa cells and mice lacking ERb (bERKO) are subfertile due to inefficient ovulation. Previous work has focused on isolated granulosa cells or cultured follicles and while informative, provides confounding results due to the heterogeneous cell types present including granulosa, theca and oocytes and exposure to in vitro conditions. Herein, we isolated preovulatory granulosa cells from WT and ERb-null mice using laser capture microdissection to examine the genomic transcriptional response downstream of PMSG (mimicking

ORGANISM(S): Mus musculus

SUBMITTER: NIEHS Microarray Core 

PROVIDER: E-GEOD-44651 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress