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Effect of GDF3 on NCCIT cells

ABSTRACT: The aim of the study was to characterize the role of GDF3 in NCCIT cells as a model of cancer stem cells. The NCCIT cells were stimulated for 3h with (1) 100ng/mL and (2) 300ng/mL GDF3, (3) 300ng/mL Nodal and (4) 100ng/mL GDF3 + 300ng/mL Nodal and compared to unstimulated control. Additionally a stable, lentiviral knockdown of GDF3 was performed in NCCIT cells and the transciption profile of these cells was compared to cells transduced with mock-virus. 5 conditions, 1 biological replicate each

ORGANISM(S): Homo sapiens

SUBMITTER: Karolina Tykwinska

PROVIDER: E-GEOD-44670 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Effect of GDF3 on NCCIT cells

Project description:The aim of the study was to characterize the role of GDF3 in NCCIT cells as a model of cancer stem cells. The NCCIT cells were stimulated for 3h with (1) 100ng/mL and (2) 300ng/mL GDF3, (3) 300ng/mL Nodal and (4) 100ng/mL GDF3 + 300ng/mL Nodal and compared to unstimulated control. Additionally a stable, lentiviral knockdown of GDF3 was performed in NCCIT cells and the transciption profile of these cells was compared to cells transduced with mock-virus.

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