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Identification of state-specific proteomic and transcriptomic signatures of microglia-derived extracellular vesicles

ABSTRACT: We performed quantitative label-free quantitative mass spectrometry (LFQ-MS) on 16 cell lysates and 16 EV lysates derived from BV2 microglia. These included four groups of BV2 cells (n=4/group) that were treated (72 hours) with either LPS (100ng/mL) to polarize to a pro-inflammatory state, IL-10 (50ng/mL) to polarize to a protective state, TGFβ (50ng/mL) to polarize to a homeostatic state or untreated controls

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Suspension Culture, Cell Culture

SUBMITTER: Juliet Santiago

LAB HEAD: Srikant Rangaraju

PROVIDER: PXD043959 | Pride | 2023-11-21

REPOSITORIES: Pride

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Identification of State-Specific Proteomic and Transcriptomic Signatures of Microglia-Derived Extracellular Vesicles.

Santiago Juliet V JV Natu Aditya A Ramelow Christina C CC Rayaprolu Sruti S Xiao Hailian H Kumar Vishnu V Kumar Prateek P Seyfried Nicholas T NT Rangaraju Srikant S

Molecular & cellular proteomics : MCP 20231111 12

Microglia are resident immune cells of the brain that play important roles in mediating inflammatory responses in several neurological diseases via direct and indirect mechanisms. One indirect mechanism may involve extracellular vesicle (EV) release, so that the molecular cargo transported by microglia-derived EVs can have functional effects by facilitating intercellular communication. The molecular composition of microglia-derived EVs, and how microglial activation states impact EV composition ...[more]

PMID: 37952696

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