Genome-wide binding profiles of KLF3 and KLF3 mutants in MEF cells

ABSTRACT: Transcription factors are often regarded as being comprised of a DNA-binding domain and a functional domain. The two domains are considered separable and autonomous, with the DNA-binding domain directing the factor to its target genes and the functional domain imparting transcriptional regulation. We have examined a typical Zinc Finger (ZF) transcription factor from the Krüppel-like factor (KLF) family, KLF3. This factor has an N-terminal repression domain that binds the co-repressor C-terminal binding protein (CtBP), and a DNA-binding domain composed of three classical (ZFs) at its C-terminus. We established a system to compare the genomic occupancy profile of wildtype KLF3 with two mutants affecting the N-terminal functional domain: a mutant unable to contact its cofactor CtBP and a muta

ORGANISM(S): Mus musculus

SUBMITTER: Jon Burdach 

PROVIDER: E-GEOD-44748 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress