Genome-wide binding profiles of an artificial zinc finger protein with or without a KLF3 functional domain

ABSTRACT: Transcription factors are often regarded as having two distinct components: a DNA binding domain (DBD) to allow binding to the regulatory region of a target gene and a trans-acting functional domain (FD) to modulate gene expression. Recently, it is becoming clear that the DBDs of transcription factors alone are incapable of providing sufficient specificity to account for the highly complex genomic structures in eukaryotes. Other regions, outside of the DBD, may play a role in transcription factor DNA binding specificity in vivo. In order to test this hypothesis, we examined a model zinc finger transcription factor, Krüppel-like factor 3 (KLF3) with a FD that recruits partner proteins for its repressive activity and a three zinc finger DBD. Previously, we showed that deletion of the entire

ORGANISM(S): Homo sapiens

SUBMITTER: WOOI FANG LIM 

PROVIDER: E-GEOD-69739 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress