Identification of novel copy number alterations by high-resolution aCGH from FFPE cutaneous melanoma samples

ABSTRACT: We investigated a cohort of 34 archival cutaneous melanoma samples by Agilent 40 kb-resolution CGH array. We found a non-random distribution of precise CNAs predictive for clinical outcome. Although most of the alterations defined in this study have been already reported, we mapped novel melanoma-specific CNAs at highest accuracy. Moreover, our data revealed distinct amplifications hotspots, some of which were validated by quantitative real-time PCR, enabling the identification of novel melanoma oncogenic candidates. Keywords: Cutaneous melanoma, Copy number alterations, Biomarkers, FFPE We examined 34 primary melanoma formalin-fixed and paraffin-embedded (FFPE) samples by using array comparative genomic hybridization (aCGH) for DNA copy number alterations (CNAs). Genomic DNA was extracte

ORGANISM(S): Homo sapiens

SUBMITTER: Toma Tebaldi 

PROVIDER: E-GEOD-45354 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress