Genome-wide probing of RNA structure reveals active unfolding of mRNA structures in vivo

ABSTRACT: We present an approach for globally monitoring RNA structure in native conditions in vivo with single nucleotide precision. This method is based on in vivo modification with dimethyl sulfate (DMS), which reacts with unpaired adenine and cytosine residues9, followed by deep sequencing to monitor modifications. Our data from yeast and mammalian cells are in excellent agreement with known mRNA structures and with the high-resolution crystal structure of the Saccharomyces cerevisiae ribosome10. Comparison between in vivo and in vitro data reveals that in rapidly dividing cells there are vastly fewer structured mRNA regions in vivo than in vitro. Even thermostable RNA structures are often denatured in cells, highlighting the importance of cellular processes in regulating RNA structure. Indeed,

ORGANISM(S): Homo sapiens

SUBMITTER: Silvi Rouskin 

PROVIDER: E-GEOD-45803 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress