High throughput screening for chemical inducers of stumpy formation in Trypanosoma brucei

ABSTRACT: During the bloodstream stage of the Trypanosoma brucei lifecycle, the parasite exists as the proliferative slender-form or the non-proliferative, transmissible, stumpy-form. The transition from the slender to stumpy-form is stimulated by a density-dependent mechanism and is important in infection dynamics, ordered antigenic variation and disease transmissibility. Here, we use a monomorphic reporter cell line in a whole-cell fluorescence-based assay to screen over 6000 small molecules from a kinase-focussed compound library for their ability to induce stumpy-like formation in a high-throughput screening programme. This identified one compound able to induce modest, yet specific, changes in gene expression indicative of a partial differentiation to stumpy forms. This not only provides a pote

ORGANISM(S): Trypanosoma brucei brucei

SUBMITTER: Alasdair Ivens 

PROVIDER: E-GEOD-46483 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress