Dataset Information


Development of Trypanosoma brucei EATRO 1125 in the host bloodstream

ABSTRACT: Trypanosoma brucei EATRO 1125 parasites were grown in adult MF1 mice with parasites harvested on day 3 post-infection ('ascend/slender') or on day 6 post-infection ('peak/stumpy'). Overall design: Three replicates each of two conditions (ascending/slender, peak/stumpy) were analysed. 'Peak' samples were each derived from infections in three individual mice; 'ascend' samples were derived from 2 individual mice infections (replicate 1 and 2) and from a pool of four infections (replicate 3), to generate sufficient material for analysis. All samples were analysed for their cell cycle status by scoring the number of kinetoplasts and nuclei in parasites and were assayed for PAD1 expression, a marker of stumpy formation.

INSTRUMENT(S): Illumina HiSeq 2000 (Trypanosoma brucei brucei)

SUBMITTER: Alasdair Ivens  

PROVIDER: GSE114810 | GEO | 2018-10-01


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A gene expression comparison of Trypanosoma brucei and Trypanosoma congolense in the bloodstream of the mammalian host reveals species-specific adaptations to density-dependent development.

Silvester Eleanor E   Ivens Alasdair A   Matthews Keith R KR  

PLoS neglected tropical diseases 20181011 10

In the bloodstream of mammalian hosts Trypanosoma brucei undergoes well-characterised density-dependent growth control and developmental adaptation for transmission. This involves the differentiation from proliferative, morphologically 'slender' forms to quiescent 'stumpy' forms that preferentially infect the tsetse fly vector. Another important livestock trypanosome, Trypanosoma congolense, also undergoes density-dependent cell-cycle arrest although this is not linked to obvious morphological t  ...[more]

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