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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

ABSTRACT: In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 . Heat shock induced GFP-marked clones ectopically expressing NetA+Fra in larvae were generated. Controls for this study included age matched wildtype third instar wing imaginal discs bearing GFP clones which were prepared in the same manner. Total RNA was extracted from dissected +NetA-Fra vs. control third instar wing imaginal discs and hybridized to the Affymetrix GeneChip Drosophila Genome 2.0.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Molly Scheel

PROVIDER: E-GEOD-47113 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression profile of Drosophila third instar larval wing imaginal discs overexpressing netrinA (netA) and frazzled (fra).

Project description:In order to analyze the global changes in gene expression resulting from induction of NetA-Fra signaling, we carried out a microarray experiment comparing Drosophila third instar wing imaginal discs in which Net+Fra had been overexpressed to age matched wild type wing imaginal discs. RNA extracted from both +NetA-Fra overexpression and wildtype third instar imaginal discs were hybridized to the Affymetrix GeneChip Drosophila Genome 2.0 .

2013-05-21 | GSE47113 | GEO

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2021-09-09 | PXD019761 | Pride

Transcriptomic analysis of third instar larval wing imaginal discs of Drosophila melanogaster corto and uL11 mutants

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae mutant for corto (cortoL1/corto420) The reference line was the w1118 genetic background of the mutant lines.

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Transcriptome analysis of control (y w) and Paip1 mutant third instar larval wing imaginal discs

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Gene expression changes in Nicd induced wing disc hyperplasia

Project description:The outcome of Notch proliferation on proliferation depends on the context. In Drosophila wing imaginal discs Notch activation causes hyperplasia despite having localized inhibitory effects on proliferation. To understand te underlying mechanisms we have used genomic strategies to identify the Notch-Su(H) target genes during wing discs hyperplasis. these data are the results from expression profiling the RNAs from hyperplastic wing discs overexpressing Nicd. Direct comparison of third instar lavae wing imaginal disc Nicd (abxUbxFLPase; Act>y>Gal4, UAS GFP; FRT82B tubGal80 with UAS-Nicd; FRT82B) vs control (abxUbxFLPase; Act>y>Gal4, UAS GFP; FRT82B tubGal80 with FRT82B ). 4 Biological replicates, the 2nd replicate was performed as a dye-swap.

2013-12-31 | E-GEOD-41428 | biostudies-arrayexpress

Expression profiles from Drosophila melanogaster scrib and lgl mutant wing imaginal disc and brain complexes at day 9 after egg deposition (AED) versus wild type at day 5 AED

Project description:mRNA microarray of Drosophila wing imaginal discs or brain complexes from lgl27S3/lglE2S31 (lgl-null), scrib1/scrib2 or wild-type 3rd instar larvae RNA was isolated from 20 pairs of wing discs/brain complexes per sample (i.e 20 individuals). Samples were from 5 day AED wildtype and 9 day AED mutant tissue Samples were prepared in triplicate from lgl27S3/lglE2S31 (lgl-null) and scrib1/scrib2 samples and in duplicate from the wild-type 3rd instar larvae

2014-01-31 | E-GEOD-48852 | biostudies-arrayexpress

Transcriptomic analysis of third instar larva wing imaginal discs deregulating Cyclin G in Drosophila melanogaster

Project description:We report here the transcriptomic analysis of Drosophila melanogaster wing imaginal discs from third instar female larvae expressing Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) under the control of the daugterless-Gal4 ubiquitous driver. The negative control was transgenic flies wearing only the daugterless-Gal4 driver.

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Genome-wide binding of Cyclin G in Drosophila melanogaster third instar larva wing imaginal discs.

Project description:We describe here the genome-wide binding sites of Cyclin G in Drosophila melanogaster wing imaginal discs from third instar female larvae. We used a transgenic line expressing a version Cyclin G deleted of the PEST domain (the 25 COOH-terminal amin-acids) and tagged by Myc under the control of the daugterless-Gal4 ubiquitous driver.

2017-07-01 | GSE99461 | GEO

Gene expression changes in Su(H) induced wing disc hyperplasia

Project description:The outcome of Notch activation of proliferation depends on cellular context. In Drosophila wing discs Notch pathway overactivation results in hyperplasia. To understand the mechanisms we have used genomic strategies to indetify the Notch-S(H) target genes directly regulated in wing disc hyperplasia. These data are the results from expression profiling the RNAs from hyperplastic wing discs overexpressing Su(H). Direct comparison of Giant third instar lavae wing imaginal disc (UAS-GFP:Su(H) expressed by the patched[559.1]-Gal4 driver) vs control (UAS-NLS-GFP expressed by the patched[559.1]-Gal4 driver). 3 Biological replicates, the 3rd replicate was performed as a dye-swap.

2013-12-31 | E-GEOD-41427 | biostudies-arrayexpress

Identification of factors involved in JAK/STAT-dependent cell competition

Project description:We report the transcriptional profiles obtained by next-generation sequencing (NGS) of FACS-purified cells from the dpp-domain of wing imaginal discs from mid-third instar Drosophila melanogaster larvae. The goals of this study are to compare NGS-derived transcriptome profiling (RNA-seq) of dpp-domain cells that have elevated JAK/STAT signaling or elevated Myc expression compared to control cells.

2019-06-21 | GSE130993 | GEO

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