High-efficiency RNA cloning enables quantitative measurement of miRNA expression by deep sequencing

ABSTRACT: MiRNA-mediated regulation depends on the stoichiometry between miRNAs and their mRNA targets. To decipher dynamic function of this complex layer, it is critical to characterize individual miRNA species within a specific cellular context. Small RNA cloning followed by deep sequencing is uniquely positioned as a genome-wide profiling method to quantify miRNA expression with potentially unlimited dynamic range and provide single-nucleotide resolution for precise miRNA classification and de novo discovery. However, significant biases introduced by RNA ligation steps in the current RNA cloning protocol often lead to inaccurate miRNA quantification by >1000-fold deviation. As a result, it has greatly hindered the broad application of this method. Here we report a highly efficient RNA cloning met

ORGANISM(S): Mus musculus

SUBMITTER: Zhaojie Zhang 

PROVIDER: E-GEOD-47858 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress