Detection of Bacillus anthracis DNA in complex soil and air samples using next-generation sequencing [NimbleGen Array data]

ABSTRACT: One of the cornerstones of an effective biodefense strategy is the ability to detect infectious agents with a high degree of sensitivity and specificity in the context of a complex sample background. The nature of the B. anthracis genome, however, renders specific detection difficult, due to close homology with B. cereus and B. thuringiensis. We therefore elected to determine the efficacy of next-generation sequencing analysis and microarrays for detection of B. anthracis in an environmental background. We applied next-generation sequencing to titrated genome copy numbers of B. anthracis in the presence of background nucleic acid extracted from aerosol and soil samples. We found next-generation sequencing to be capable of detecting as few as 10 genomic equivalents of B. anthracis DNA p

ORGANISM(S): Bacillus anthracis str. Ames

SUBMITTER: Kevin McLoughlin 

PROVIDER: E-GEOD-48316 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress