Transcription profiling of human thyroid cells exposed to INF-gamma and IL1-beta

ABSTRACT: Normal primary thyroid cells were incubated with , 100 IU/ml IFN-gamma, 50 IU/ml IL1-beta, or a combination of both IFN-gamma and IL1-beta for 24 or 72 hours. The experiment was repeated 5 times using thyroid cells from 5 different patients. RNA expression was analyzed using Affymetrix HG_U133A arrays for 3 of the thyroids, and HG_U133A_2.0 (small version of HG_U133A) arrays for 2 of the thyroids. Experiment Overall Design: Normal primary thyroid cells were incubated with , 100 IU/ml IFN-gamma, 50 IU/ml IL1-beta, or a combination of both IFN-gamma and IL1-beta for 24 or 72 hours. The experiment was repeated 5 times using thyroid cells from 5 different patients. One array was hybridized per sample (20 samples). Experiment Overall Design: The probe-set intensities with accompanying statistical analysis (2-way ANOVA) and the associated README file are included as Supplementary files.

ORGANISM(S): Homo sapiens

SUBMITTER: Rork Kuick 

PROVIDER: E-GEOD-5054 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress