A reference map of the human proinsulin biosynthetic interaction network
Ontology highlight
ABSTRACT: A monoclonal antibody specific for human proinsulin (20G11) was generated and utilized for affinity purification mass spectrometry (AP-MS) for unbiased profiling of proinsulin protein:protein interactions in human islets. Half of each islet prep was treated with the cytokines IL1-beta and IFN-gamma. Immunoprecipitated proinsulin or control IgG immunoprecipitates from human islets were then subjected to state of the art mass spectrometry revealing a rich proinsulin biosynthetic network that is remarkably conserved across a diverse group of donors.
INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive
ORGANISM(S): Homo Sapiens (ncbitaxon:9606)
SUBMITTER: 
Pamela Itkin-Ansari
PROVIDER: MSV000084057 | MassIVE | Wed Jul 03 15:25:00 BST 2019
SECONDARY ACCESSION(S): PXD014476
REPOSITORIES: MassIVE
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