Lipopolysaccharide-induced microRNA-146a targets CARD10 and involves in angiogenesis of human umbilical vein endothelial cells

ABSTRACT: This study was designed to explore the role of miRNA-146a (miR-146a) and its target genes in the endothelial cells. In this study we have demonstrated that lipopolysaccharide (LPS) induced upregulation of miR-146a in the human umbilical vein endothelial cells (HUVEC) and the induction was blocked by the silencing of the toll-like receptors (TLRs) adaptor molecules MyD88 and nonspecific NF-M-NM-:B inhibitor BAY 11-7082. In addition, knockdown of miR-146a by transfection of the locked nucleic acid (LNA)-antimiR-146a significantly decreased the increased cell migration and tube formation induced by LPS. A combined analysis of the bioinformatics miRanda algorithms and the whole genome expression microarray of the immunoprecipitated Ago2 ribonucleoprotein complex identified 14 transcripts as th

ORGANISM(S): Homo sapiens

SUBMITTER: Ching-Hua Hsieh 

PROVIDER: E-GEOD-50619 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress