Sox2 promotes malignancy in glioblastoma by regulating plasticity and astrocytic differentiation

ABSTRACT: Making use of a previously described isogenic cancer stem cells and serum differentiated cultures we show that Sox2 controls developmental stated specific programs in glioblastoma. Glioblastoma cells were cultured as control and with SOX2 knockdown to identify the scope of SOX2 interactions. The SOX2 knockdown were accomplished using two knockdown technologies. The knockdown cells were compared to controls, early passage, and scrambled controls. For Sox2 knockdown in low passage 10% FBS cells, the following oligonucleotides targeting human SOX2 coding sequence, or non-silencing control sequence, were cloned into BLOCK-iT Pol II miR RNAi expression vectors (Invitrogen), before cell transfection into GBM monolayer cells using Lipofectamine-2000 (Invitrogen): Sox2miRNA1R:5’CCTGTGCATGGGCTGTCT

ORGANISM(S): Homo sapiens

SUBMITTER: David Cherba 

PROVIDER: E-GEOD-51441 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress