Unknown,Transcriptomics,Genomics,Proteomics

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Prenatal stress-induced programming of genome-wide promoter DNA methylation in 5-Htt deficient mice

ABSTRACT: The serotonin transporter (5-HTT) gene-linked polymorphic region has been suggested to play a modulatory role in mediating the effects of early-life stress on psychopathology rendering carriers of the low-expression short (s)-allele more vulnerable to environmental adversity in later life. Here we analyzed the effects of prenatal stress (PS), 5-Htt genotype, and an interactin of both on DNA methylation in the hippocampi of female C57BL/6 mice. Here, we applied Methylated DNA ImmunoPrecipitation (MeDIP) in a maternal restraint stress paradigm to perform a global promoter DNA methylation screen: Hippocampal DNA of wild type and 5-Htt +/- mice in stressed and control environments were analyzed to define genotype- (G) and environment-dependent (E) as well as GxE-interactive effects on promoter DNA methylation in the brain region, where marked effects were expected. MeDIP-based promoter DNA methylation screen

ORGANISM(S): Mus musculus

SUBMITTER: Claus Scholz

PROVIDER: E-GEOD-51634 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Prenatal stress-induced programming of genome-wide promoter DNA methylation in 5-HTT-deficient mice.

Schraut K G KG Jakob S B SB Weidner M T MT Schmitt A G AG Scholz C J CJ Strekalova T T El Hajj N N Eijssen L M T LM Domschke K K Reif A A Haaf T T Ortega G G Steinbusch H W M HW Lesch K P KP Van den Hove D L DL

Translational psychiatry 20141021

The serotonin transporter gene (5-HTT/SLC6A4)-linked polymorphic region has been suggested to have a modulatory role in mediating effects of early-life stress exposure on psychopathology rendering carriers of the low-expression short (s)-variant more vulnerable to environmental adversity in later life. The underlying molecular mechanisms of this gene-by-environment interaction are not well understood, but epigenetic regulation including differential DNA methylation has been postulated to have a ...[more]

PMID: 25335169

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