Integrated approach to explore the mechanisms of aromatase inhibition and recovery on fathead minnow ovaries

ABSTRACT: Aromatase, a member of the cytochrome P450 superfamily, is a key enzyme in estrogen synthesis and is responsible for the aromatization of androgens into estrogens. Here, we used an integrated approach to better understand the effects of aromatase inhibition as well as the mechanisms involved in the adaptation and recovery process. We exposed female fathead minnows (Pimephales promelas) to 30 μg/L of a model aromatase inhibitor, fadrozole, during 8 days (exposure phase). Fish were then held in clean water for 8 more days (recovery phase). Samples were collected at 1, 2, 4, and 8 days of both the exposure and the recovery phase. We used transcriptomics, metabolomics, and network inference to understand changes and infer connections at the transcript and metabolite level in the ovary. Other

ORGANISM(S): Pimephales promelas

SUBMITTER: Tanwir Habib 

PROVIDER: E-GEOD-51961 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress