Global indiscriminate methylation in cell-specific gene promoters following reprogramming into human induced pluripotent stem cells

ABSTRACT: Genome wide DNA methylation profiling of somatic and pluripotent cells from different lineages (mesoderm, endoderm and parthenogenetic germ cells) The Illumina Infinium 27k Human DNA methylation Beadchip v1.2 was used to obtain DNA methylation profiles across approximately 27,000 CpGs. Samples included 1 Human ES cell line, 2 beta cells, 2 beta-iPS cells, 1 fibroblast, 2 fibroblast-iPS cells, 2 parthenogenetic cells and 3 parthenogenetic-iPS cells. Molecular reprogramming of somatic cells into human induced pluripotent stem cells (iPSCs) is accompanied by extensive changes in gene expression patterns and epigenetic marks. To better understand the link between gene expression and DNA methylation, we have profiled human somatic cells from different embryonic cell types (endoderm, mesoderm an

ORGANISM(S): Homo sapiens

SUBMITTER: Jonathan Nissenbaum 

PROVIDER: E-GEOD-52238 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress