C/EBPM-NM-1 poises B cells for rapid reprogramming into iPS cells

ABSTRACT: C/EBPM-NM-1 induces transdifferentiation of B cells into macrophages at high efficiencies and enhances reprogramming into induced pluripotent stem cells (iPSCs) when co-expressed with Oct4, Sox2, Klf4 and Myc (OSKM). However, how C/EBPM-NM-1 accomplishes these effects is unclear. We now found that transient C/EBPM-NM-1 expression followed by OSKM activation induces a 100 fold increase in iPSC reprogramming efficiency, involving 95% of the cells. During this conversion pluripotency and epithelial-mesenchymal transition genes become dramatically up-regulated and 60% of the cells express Oct4 within 2 days. C/EBPM-NM-1 acts as a pathbreaker since it transiently makes the chromatin of pluripotency genes more accessible to DNase I. It also induces the expression of the dioxygenase Tet2 and prom

ORGANISM(S): Mus musculus

SUBMITTER: Bruno Di Stefano 

PROVIDER: E-GEOD-52397 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress