Enhanced Direct Conversion into Hepatocyte-like Cells by Accelerated Sequential Epigenetic Events using Additional Stem Cell Factors

ABSTRACT: Recent studies have shown that defined sets of transcription factors could directly convert fibroblasts into induced hepatocytes (iHeps). However, the underlying mechanism of direct conversion process toward a hepatic lineage is largely unknown. Here, we report that the direct conversion kinetics from fibroblasts into iHeps throughout screening multiple additional factors that potentially rescue the delayed kinetics of MET and hepatic program. Mouse embryonic fibroblasts (MEFs) were efficiently converted into iHeps in the presence of c-Myc and Klf4 (CK), the activators for MET process, with the previously defined sets of hepatic transcription factors, resulting in remarkably improved generation of iHeps. Furthermore, in the presence of CK, Hnf4? alone could convert fibroblasts into iHeps w

ORGANISM(S): Mus musculus

SUBMITTER: Kyungtae Lim 

PROVIDER: E-GEOD-52566 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress