Dissecting engineered cell types and enhancing cell fate conversion via CellNet

ABSTRACT: Engineering clinically relevant cells in vitro holds promise for regenerative medicine, but most protocols fail to faithfully recapitulate target cell properties. To address this, we developed CellNet, a network biology platform that determines whether engineered cells are equivalent to their target tissues, diagnoses aberrant gene regulatory networks, and prioritizes candidate transcriptional regulators to enhance engineered conversions. Using CellNet, we improved B cell to macrophage conversion, transcriptionally and functionally, by knocking down predicted B cell regulators. Analyzing conversion of fibroblasts to induced hepatocytes (iHeps), CellNet revealed an unexpected intestinal program regulated by the master regulator Cdx2. We observed functional engraftment of mouse colon by iHep

ORGANISM(S): Mus musculus

SUBMITTER: Hu Li 

PROVIDER: E-GEOD-59037 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress