Cell cycle positioning drives heterogeneity within the pluripotent stem cell compartment

ABSTRACT: Heterogeneity in pluripotent cells marks a metastable state where cells may drift between native and lineage-primed populations. While the role for these heterogeneities are unclear, they may reflect the dynamic equilibriums of signaling networks and have a direct effect on differentiation potentialities. Here, we report the role of the cell cycle in establishing heterogeneity of human pluripotent stem cells. By utilizing the FUCCI cell cycle indicator system coupled to fluorescent activated cell sorting (FACS), we have uncovered that the cell cycle drives heterogeneity at the epigenetic, transcriptional and post-transcriptional levels. Our data show widespread dynamics in 5-hydroxymethylcytosine (5hmC) during the cell cycle. Furthermore, transcript profiling by RNA-sequencing identif

ORGANISM(S): Homo sapiens

SUBMITTER: Stephen Dalton 

PROVIDER: E-GEOD-53481 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress