ERK Oscillation-Dependent Gene Expression Patterns and Deregulation By The Stress-Response

ABSTRACT: Studies were undertaken to determine whether oscillatory behavior in the extracellular signal regulated kinase (ERK) pathway results in unique gene regulation patterns. Microarray analysis was performed on three subcloned populations of human keratinocytes with distinct ERK signaling/oscillation phenotypes. Microarray analysis identified 45 genes that overlapped between 2 subclones with oscillation phenotypes but not in the subclone which is non-oscillatory. Transcription factor networks revealed a role for MED1 in mediating ERK oscillation-dependent gene expression, which was confirmed with Western blot analysis. Further experimentation confirmed a role for p38 in the mediation of MED1 phosphorylation and ERK oscillatory behavior. hTERT-immortalized normal human keratinocytes (provided

ORGANISM(S): Homo sapiens

SUBMITTER: Katrina Waters 

PROVIDER: E-GEOD-55823 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress