Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Expression data from HT29 intestinal epithelial cell line

ABSTRACT: The study recapitulates, through in vitro micropatterned co-cultures, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function during HIV infection. The co-culture method simplifies observation/monitoring of the two cell types and is particularly suited for laser microdissection-based retrieval of the epithelial cells for downstream gene expressions studies. Microarrays were used to characterize changes in gene expression in HT29 epithelial cells co-cultured with Jurkat T-lymphocytes in the presence and absence of HIV infection Micropatterned co-cultures were generated of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (HT-29) cells whereby both cell types were positioned on the same surface in a price spatial configuration (micropattern). HT29 celsl were then isolated by laser capture microdissection and used for RNA extraction and hybridization on Affymetrix U133 Plus2 microarrays.

ORGANISM(S): Homo sapiens

SUBMITTER: Michael George 

PROVIDER: E-GEOD-56090 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Micropatterned co-cultures of T-lymphocytes and epithelial cells as a model of mucosal immune system.

Stybayeva Gulnaz G   Zhu He H   Ramanculov Erlan E   Dandekar Satya S   George Michael M   Revzin Alexander A  

Biochemical and biophysical research communications 20090131 3

Gut-associated lymphoid tissue is a major target and reservoir of human immunodeficiency virus (HIV)-infected T-cells. Our studies seek to recapitulate, in vitro, interactions between HIV-infected T-lymphocytes and intestinal epithelial cells in order to investigate the mechanisms underlying the disruption of normal epithelial cell and barrier function. Here, we describe a novel approach for creating co-cultures of healthy or HIV-infected T-lymphocytes (Jurkat) and human intestinal epithelial (H  ...[more]

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