Interrelationships between replication infidelity and genome composition

ABSTRACT: An enduring issue in evolutionary and cancer biology is how replication infidelity influences genome composition and vice versa. Here we examine this issue by sequencing the genomes of diploid budding yeast strains that are either mismatch repair (MMR) proficient or deficient and encode wild type or mutator variants of the three major nuclear DNA replicases. Analysis of over 43,000 mutations that accumulated in the absence of selective pressure demonstrates that the nuclear DNA replication machinery generates less than one mismatch per genome and in combination with MMR, achieves a genome-wide per base error rate of 1.7 x 10-10. Absent both MMR and purifying selection, replication error patterns strongly depend on replication origin proximity, replication fork direction, and the local DNA

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Scott Lujan 

PROVIDER: E-GEOD-56939 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress