Characterization of extended transcripts by RNA-Seq suggests that Rrp6 influences Nrd1-dependent termination

ABSTRACT: RNAPII is responsible for transcription of protein-coding genes and short, regulatory RNAs. In Saccharomyces cerevisiae, termination of RNAPII-transcribed RNAs ≤1000 bases requires the NNS complex (comprised of Nrd1, Nab3, and Sen1) processing by the exosome, and the nuclear specific catalytic subunit, Rrp6. It has been shown that Rrp6 interacts directly with Nrd1, but whether or not Rrp6 is required for NNS-dependent termination is unclear. Loss of Rrp6 function may result in extension (or inhibition of termination) of NNS-dependent transcripts, or Rrp6 may only function after the fact to carry out RNA 3’ end processing. Here, we performed in-depth differential expression analyses and compare RNA-sequencing data of transcript length and abundance in cells lacking RRP6 to previously publis

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Amber Mosley 

PROVIDER: E-GEOD-57155 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress