Deep sequencing of small RNA facilitates tissue and sex associated microRNA discovery in zebrafish

ABSTRACT: The role of microRNAs in gene regulation has been well established. The extent of miRNA regulation also increases with increasing genome complexity. Though the number of genes appear to be equal between human and zebrafish, substantially less microRNAs have been discovered in zebrafish compared to human (Release 19). It appears that most of the miRNAs in zebrafish are yet to be discovered. We sequenced small RNAs from brain, gut, liver, ovary, testis, eye, heart and embryo of zebrafish. In brain, gut and liver sequencing was done in male and female separately. Majority of the sequenced reads (16-62%) mapped to known miRNAs, with the exception of ovary (5.7%) and testis (7.8%). Using the miRNA discovery tool (miRDeep2), we discovered novel miRNAs from the un-annotated reads that ranged fro

ORGANISM(S): Danio rerio

SUBMITTER: Vivek Tanavde 

PROVIDER: E-GEOD-57169 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress