Identification and Characterization of MicroRNAs in Zebrafish Spermatozoa by Illumina Sequencing

ABSTRACT: MicroRNAs (miRNAs) are involved in nearly every biological process examined to date. Mounting evidence show that some spermatozoa specific miRNAs play important roles in the regulation of spermatogenesis and germ cells development, but little is known of the exact identity and function of miRNA in sperm cells or their potential involvement in spermatogenesis and germ cells development. Here, we investigated the spermatozoa miRNA profiles using illumina deep sequencing combined with bioinformatic analysis using zebrafish as a model system. Deep sequencing of small RNAs yielded 12 million raw reads from zebrafish spermatozoa. Analysis showed that the noncoding RNA of the spermatozoa included tRNA, rRNA, snRNA, snoRNA and miRNA. By mapping to the zebrafish genome, we identified 400 novel and

ORGANISM(S): Danio rerio

SUBMITTER: kun jia 

PROVIDER: E-GEOD-61984 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress