Identification and characterization of wilt and salt stress-responsive microRNAs from chickpea by high-throughput sequencing.

ABSTRACT: Purpose: To identify Fusarium wilt and salt-responsive miRNAs at genome wide level in Chickpea. Results: A total of 12,135,571 unique reads were obtained. In addition to 122 conserved miRNAs belonging to 25 different families, 59 novel miRNAs along with their star sequences were identified. Four legume specific miRNAs, miR5213, miR5232, miR2111 and miR2118 were found in all the libraries. The Poly (A) tailing assay based qRT-PCR was used to validate eleven conserved and five novel miRNAs. miR530 was highly up regulated in response to fungal infection and targets zinc knuckle and microtubule-associated proteins. Many miRNAs responded in a similar fashion under both biotic and abiotic stresses indicating a cross talk between the pathways involved in regulating these stresses. The potential t

ORGANISM(S): Cicer arietinum

SUBMITTER: Gopal Joshi 

PROVIDER: E-GEOD-57857 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress