Transcription profiling of mouse models of sepsis cecal ligation and puncture and tracheal instillation of P. aeruginosa reveals bcl-2 overexpression modulates transcription responses in vivo

ABSTRACT: Background: We hypothesized that spleen microarray gene expression profiles analyzed with contemporary pathway analysis software would provide molecular pathways of interest and target genes that might help explain the affect of bcl-2 on improving survival during sepsis. Methods: Two mouse models of sepsis, cecal ligation and puncture and tracheal instillation of Pseudomonas aeruginosa, were tested in both wild-type mice and mice that overexpress bcl-2. Whole spleens were obtained 6 hours after septic injury. DNA microarray transcriptional profiles were obtained using the Affymetrix 430A GeneChip, containing 22,690 elements. Ingenuity Pathway Analysis software was used to construct hypothetical transcriptional networks that changed in response to sepsis and expression of the bcl-2 trans

ORGANISM(S): Mus musculus

SUBMITTER: J Perren Cobb 

PROVIDER: E-GEOD-5811 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress