Molecular profiling of single circulating tumor cells with diagnostic intention

ABSTRACT: About 230 clinical trials currently explore the role of circulating tumor cell (CTC) analysis for therapy decisions, but no assays enable comprehensive molecular characterization of CTCs with diagnostic precision. We therefore combined a workflow for CTC enrichment and isolation with 100% purity with a non-random whole genome amplifiation method for single cells and applied it to 510 single CTCs and 189 leukocytes of 66 breast cancer patients. We defined a genome integrity index (GII) to identify cells suited for molecular chracterization by different molecular assay in more than 90% of single cells, such as diagnostic profiling for point mutations, gene amplifications and whole genomes of single cells. The high reliability on clinical samples enabled assessing the molecular heterogeneity

ORGANISM(S): Homo sapiens

SUBMITTER: Zbigniew Czyz 

PROVIDER: E-GEOD-58192 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress