X-chromosome dynamics revealed by the RNA interactome and chromosomal binding of CTCF

ABSTRACT: CTCF is a master regulator that plays a role in genome architecture and gene expression. A key aspect of CTCF’s mechanism involves bringing together distant genetic elements for intra- and inter-chromosomal interactions. Evidence from epigenetic processes, such as X-chromosome inactivation (XCI), suggests that CTCF may carry out its functions through interacting RNAs. Using genome-wide approaches to investigate the relationship between CTCF’s RNA interactome and its epigenomic landscape, here we report that CTCF interacts with thousands of transcripts in mouse embryonic stem cells (mESC), many in close proximity to CTCF’s genomic binding sites. Biochemical analysis demonstrates that CTCF is a high-affinity RNA binding protein that contacts RNA directly and specifically. In the XCI model, C

ORGANISM(S): Mus musculus

SUBMITTER: Johnny Kung 

PROVIDER: E-GEOD-58242 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress