Transcription profiling of human angiomyolipoma xenograft tumours grown in mice after estrogen treatment

ABSTRACT: SV7tert AML cells were obtained from ATCC and cultured in Dulbecco's modified essential medium (DMEM), glutamine (4mmol) and 10% foetal bovine serum (FBS). Two million SV7tertAML cells were subcutaneously injected into nude mice either with or without subcutaneous oestrogen pellets (n=4 per group); oestrogen was added using 0.36mg 60 day release oestrogen pellets implanted sub-cutaneously. Mice were housed in pathoflex isolators at 26°C, on 12 hour light / dark cycles. Irradiated RB2 diet and autoclaved water provided ad libertum. All oestrogen treated mice inoculated with SV7tertAML cells developed subcutaneous tumours which required termination of the animals at six weeks due to tumour load. These primary tumours were removed and fragments placed subcutaneously in further nude mice whi

ORGANISM(S): Homo sapiens

SUBMITTER: Simon Johnson 

PROVIDER: E-GEOD-5868 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress