Identification of candidate genes involved in fructooligosaccharides (FOS) utilization by Lactobacillus paracasei

ABSTRACT: A shotgun microarray-based approach was used to identify candidate genes encoding the FOS utilization pathway in L. paracasei 1195. Differential expression profiles between cells grown on FOS and glucose provided a basis for identifying genes specifically induced by FOS. In addition, transcriptional analysis of cells grown on FOS or FOS + glucose allowed us to investigate the effect of catabolite repression on the expression of the FOS-induced genes. For sugar induction experiment (FOS vs. glucose; FOS vs. fructose), L. paracasei were grown in modified MRS (mMRS) basal medium to a final OD625 nm of ca. 0.3, at which point all of the residual sugars in the medium were consumed (based on HPLC analysis of the supernatants). The culture was then divided into equal portions, where FOS or glu

ORGANISM(S): Lactobacillus paracasei

SUBMITTER: Andrew Benson 

PROVIDER: E-GEOD-5890 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress