Multiple Low-dose Challenges in a Rhesus Macaque AIDS Vaccine Trial Result in an Evolving Host Response that affects Protective Outcome

ABSTRACT: Using whole-blood transcriptional profiling, we investigated differences in the host response to vaccination and challenge in a rhesus macaque AIDS vaccine trial. Samples were collected from animals prior to and after vaccination with live irradiated vaccine cells secreting the modified endoplasmic reticulum chaperone gp96-Ig loaded with SIV peptides, either alone or in combination with a SIV-gp120 protein boost. Additional samples were collected following multiple low-dose rectal challenge with SIVmac251. Animals in the boosted group had a 73% reduced risk of infection. Surprisingly, few changes in gene expression were observed during the vaccination phase. Focusing on post-challenge comparisons, in particular for protected animals, we identified a host response signature of protection co

ORGANISM(S): Macaca mulatta

SUBMITTER: Michael Katze 

PROVIDER: E-GEOD-59068 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress